(in production)



This study was performed to determine the conjunctival microbiota of Persian cats with and without nasolacrimal duct obstruction (NLDO). 

Animals studied

Twenty-five Persian cats: 15 with bilateral NLDO (Group A) and 10 with no NLDO (Group B).


All fifty eyes were assessed. Sterile swab applicators were used for the collection of specimens, which were cultured. PCR was performed on conjunctival swab and blood samples for the detection of Mycoplasma spp. and feline herpesvirus 1(FHV-1), respectively.


FHV-1 was detected in two cats in Group A. Twelve eyes from Group A and four from Group B were Mycoplasma spp. positive based on the PCR results. Moreover, fungal culture was positive in six eyes from Group A and three eyes from Group B. The dominant fungus isolated was Aspergillus spp. (6 out of 11 fungal isolates). Other isolated fungi were Alternaria spp. and Cladosporidium spp. Twenty-three eyes had positive bacterial culture in Group A, while twelve eyes were positive in Group B. The most commonly isolated bacteria was Staphylococcus epidermidis (15 out of 38 bacterial isolates). β- hemolytic Streptococcus spp., Corynebacterium spp., and Staphylococcus aureus were also isolated in similar proportions in both groups. Escherichia coli was also present in both groups.


Results of this study revealed same isolated fungal and bacterial spp. and in similar proportions in Persian cats with and without NLDO.